The effect of ketorolac on biofilm of Staphylococcus epidermidis isolated from post-cataract endophthalmitis

نویسندگان

  • Silvia Rossetti
  • Leonardo D’Alessandro
  • Fernando Pellegrino
  • María Alejandra Carrasco
چکیده

PURPOSE The purpose of this study is to investigate the effect of ketorolac on biofilm formation of Staphylococcus epidermidis isolated from patients with post-cataract endophthalmitis. METHODS Forty S. epidermidis strains isolated from postoperative endophthalmitis were used for this study. Biofilms were grown on microtitre plates for 24 h, dyed, and stained with crystal violet. The mean optical density (OD) and the OD ratio (ODr = OD of the treated biofilm/OD of the untreated biofilm) were used for quantification. The biofilms were incubated with 13 mM ketorolac and without ketorolac for controls. RESULTS The biofilm ODs of the S. epidermidis isolates untreated and treated with ketorolac were significantly different (0.335 ± 0.06 versus 0.158 ± 0.03, respectively; mean ± SD; P < 0.001). Ketorolac reduced S. epidermidis biofilm formation by 47.6 %. CONCLUSIONS Ketorolac, at a concentration of 13 mM, significantly reduces the formation of biofilm by strains of S. epidermidis that caused endophthalmitis.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Endophthalmitis in the Tertiary Referral Center in Iran; Etiology and Causative Organisms

Background: Endophthalmitis is a severe and sight-threatening complication of ocular surgery or ocular trauma. The identification of common types and causative organisms is essential for prevention and management. Objective: The current study describes the prevalence of various types of endophthalmitis and the causative microbiological spectrum of each ty...

متن کامل

بررسی اثـر نانـوذرات نقـره بر بیـوفیلم‌های ناشی از استافیلوکوکوس اپیدرمیدیس

Background and Objective: Staphylococcus epidermidis produces extracellular polysaccharide which is known as a biofilm. Biofilm is highly effective in establishing of this bacterium infections and can be formed on medical devices that are used in the body. The purpose of this study was to evaluate the effects of silver colloidal nanoparticles on bacterial growth and biofilm form...

متن کامل

بررسی الگوی مقاومت آنتی‌بیوتیکی و تولید بیوفیلم در ایزوله‌های استافیلوکوکوس‌اورئوس و استافیلوکوکوس‌اپیدرمیدیس جداشده از عفونت‌های بیمارستانی شهر تهران، در سال 1395

Background and Aim: Staphylococci are common pathogens of humans and livestock that able to produce a wide range of diseases. Staphylococcus epidermidis and Staphylococcus aureus are the important factors for biofilm production in patients. This study was designed to determine the ability of&nbsp; biofilm production and the resistance pattern of Staphylococcus epidermidis and Staphylococcus aur...

متن کامل

Isolation, vancomycin resistance and biofilm production of Staphylococcus epidermidis from patients with conjunctivitis, corneal ulcers, and endophthalmitis.

The infection frequency associated to bacterial conjunctivitis, corneal ulcers (CU), and endophthalmitis was studied along a five years period. The isolation and identification of microorganisms were performed by culture-based methods and biochemical test respectively. Also, a nested PCR to detect gram-negative and gram-positive bacteria in the clinical samples was assayed. Nested PCR was a mor...

متن کامل

Fluorescent amplified fragment length polymorphism (FAFLP) genotyping demonstrates the role of biofilm-producing methicillin-resistant periocular Staphylococcus epidermidis strains in postoperative endophthalmitis

BACKGROUND An observational case series was used to study the virulence characteristics and genotypes of paired Staphylococcus epidermidis isolates cultured from intraocular samples and from periocular environment of patients with postcataract surgery endophthalmitis. METHODS Eight S. epidermidis isolates were obtained from three patients (2 from patients #1 and 2 and 4 from patient #3) whose...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره 2  شماره 

صفحات  -

تاریخ انتشار 2012